Beacon Lesson Plan Library

Glucose Factory

Jacqueline Roberts
Bay District Schools


Plants use chemicals from the environment and energy from the sun to produce their own food. The food they produce is glucose. Students determine through laboratory activity the presence or absence of glucose in a variety of plant leaves and stems.


Selects and uses appropriate instruments, technology, and techniques to measure quantities in order to achieve specified degrees of accuracy in a problem situation.

The student knows that investigations are conducted to explore new phenomena, to check on previous results, to test how well a theory predicts, and to compare different theories.


MATERIALS: ( Lab set-up is for one lab and one variety of stem and leaf.)

- Safety Goggles (all students)
- Aprons (all students)
- Masking tape
- Markers
- Beaker, 600ml. (1)
- Graduated Cylinder, 10 ml. (1)
- Test tubes (4)
- Test tube rack (1)
- Test tube holder(1)
- Funnel(1)
- Hot plate (1)
- Benedict Solution (4 ml per group)
- White Corn syrup
- Knife
- Access to water (pitcher)
- Paper towels

* * Note: If teacher wants to test more than one variety of leaf and stem, teacher should increase Benedict solution and have students wash test tubes between experiments.


Johnson, George B., [Biology: Visualizing Life]. Holt, Rhinehart and Winston, Inc., Orlando, Florida, 1994 and 1998.

Kaskel, Albert., Hummer, Paul J. and Daniel, Lucille. [Biology: An Everyday Experience]. Charles E. Merrill Publishing Co., Columbus, Ohio, 1988.

Strauss, Eric. and Lisowski., [Biology: The Web of Life]. Scott Foresman-Addison Wesley Publishing Co., Atlanta, Georgia, 1998.

[Encyclopedia Britannica]. (hardcopy and/or online)

Video Series: [Science in Action: Photosynthesis and Cellular Respiration], Holt, Rhinehart and Winston Inc., 1991.


- Review Calvin Cycle, photosynthesis and the effect of Benedicts solution on glucose.
- Know how to set up cooperative learning group.
- Take inventory of material and supplies.


- Teacher reviews Calvin Cycle.
- Teacher reviews definition of photosynthesis.
- Teacher demonstrates experiment and emphasizes that test tube three and four are the control test tubes.
- Teacher should CAUTION students on safety procedures i.e.
a. Safety goggles and laboratory aprons should be worn through out the investigation.
b. Benedicts solution is a skin irritant. If it comes in contact with the skin it must be washed away immediately.
- Teacher reviews clean-up procedures with students. (Clean up is everyone's responsibility)

- Students divide into groups consisting of 4 students per group.
- Students select:
a. Leader - is responsible for coordinating group activities.
b. Secretary - reads the lab out loud to group and maintains the data chart.
c. Materials Retriever- is responsible for gathering equipment, chemicals and plant parts
d. Materials Manager - is responsible for preparing the experiment.

1. Student (secretary) reads lab to group.
2. Students prepare a hypothesis to determine the presence or absence of glucose in leaves and stems.
3. Student (secretary) prepares the data chart. See Attachment.
4. Student (materials retriever) gathers all necessary materials for the lab.
5. Student (materials manager) -sets-up- lab according to directions read by the secretary.

1. Student places masking tape on four test tubes.
2. Student labels these test tubes one through four.
3. Student measures 4 ml of Benedicts solution in the 10 ml graduated cylinder.
4. Student chop leaves and stems in small pieces.
5. Student prepares a hot water bath. (600ml beaker about one-half full of water and placed on a hot plate.
6. Test tube number 1 - Student combines 1 ml of plant leaves and 1 ml of Benedicts solution in test tube number one.
7. Test tube number 2 - Student combines 1 ml of plant stems and 1 ml of Benedicts solution in test tube number two.
8. Test tube number 3 - Student combines 1 ml of corn syrup and 1 ml of Benedicts solution in test tube number three.
9. Test tube number 4 - Student combines 1 ml of water and 1 ml of Benedicts solution in test tube number four.
10. Student gently places all test tubes into the hot water bath.
11. Students observe the test tubes for five minutes.
12. At the end of five minutes the student (secretary) records on the data sheet the observable results. See attachment.
13. Student writes a conclusion.


Laboratory rubric

Attached Files

Data Rubric Laboratory Rubric     File Extension: pdf

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